主讲内容：Arboviruses naturally circulate between vertebrate hosts and an arthropod vectors. Viral acquisition from an infected mammalian host by a vector is one of essential steps in their lifecycles. However, little information is available regarding the molecular mechanisms underlying the viral acquisition by arthropod vectors. Here, we will introduce multiple mechanisms in mosquito-borne viral acquisition, including: 1) A gut commensal bacteria promote mosquito susceptibility to arboviruses by blood meal; 2) Nonstructural protein-1 (NS1), which is abundantly secreted into the serum of an infected host, is a key factor for flavivirus acquisition from infected hosts to fed mosquitoes; 3) the hematophagous nature of mosquitoes contributes to arboviral acquisition after a blood meal, via suppressing antiviral innate immunity by activating the GABAergic pathway. Altogether, our studies demonstrate that arboviruses smartly utilize multiple materials, either produced in their vertebrate phases or existing in vectors, to enhance their acquisition by vectors, therefore facilitating arbovirus survival in nature.